SpliceCraft

Use it when

• You design plasmids, primers, or Golden Braid/MoClo assemblies and need an account-free local workbench because the sequences are confidential.
• You want a coding agent or shell script to drive a cloning-design workflow, from primer design through simulated digestion and assembly, over a local API.

What it solves

Not the fit when

• Genome-scale sequence analysis, read mapping, protein structure prediction, or LIMS inventory management.
• A substitute for expert review, experimental validation, institutional biosafety approval, or verification of primers and assembled sequences before wet-lab use.
• Native Windows braille rendering is CI-tested but not confirmed on real hardware; ARM64 installs require a C toolchain for primer3-py.
• Online BLAST and agent web lookups are disabled by default and send sequence-related data externally when explicitly enabled.
• NGS read alignment at scale
• protein structure prediction
• wet-lab inventory or LIMS management

Install

pipx install splicecraft

Invoke

splicecraft myplasmid.gb to open a local GenBank file, splicecraft L09137 to fetch from NCBI, or splicecraft --agent to expose the local JSON API for scripting

Alternatives

No reviewed alternatives recorded yet.